This study was devised to observe the cytotoxic activities of the petroleum ether extract of Scirpus Maritimus (crude extract) and its partially purified fraction from silicic acid column chromatography(acetone fraction) against mouse
leukemic
cell (L1020), hepatoma cell (Hep G2), human rectum (HRT-18) and colon (HT-29) cancer cells, in vitro.
Each cell line was cultured in medium containing serial concentration of the crude extract or acetone fraction for 72 hours. And then the growth rate, dose-response and morphological change of each cell were determined.
@ES The results obtained are summarized as follows,
@EN 1. The doubling times of L1210, HRT-18, HT-29 and Hep G2 cells were about 12,20,22 and 40 hours, respectively.
2. The growth inhibition rates of L1210, HRT-18, HT-29 and Hep G2 cells were gradually increased in proportion to the concentration of the crude extract or acetone fraction in culture
medium.
3. The cytotoxic activity of acetone fraction on the each cell line was more potent than that of the crude extract.
4. The growth inhibition rates of the cells treated with crude or acetone fraction were shown the difference among the each cell and greatest in the L1210 cell.
5. The numbers and size of HRT-18 cultured in medium containing acetone fraction were significantly reduced and deformed compared with the control group and the effect of actone fraction was more potent than that of the crude extract.
In view of the results, there are contained the growth inhibitory substance on the several cancer cells in the acetone fraction of Scirpus Maritimus, it could be suggested that active compound is glycolipid.
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